Indian scientists develop a quick Saline Gargle RT-PCR test for Covid-19

<p>
In a major breakthrough, scientists of Nagpur-based National Environmental Engineering Research Institute (NEERI) have developed a simple 'Saline Gargle RT-PCR Method' for testing COVID-19 samples which generates a result within three hours and is cheaper than the existing methods that are in use, according to a statement issued by the Ministry of Science and Technology.</p>
<p>
Dr. Krishna Khairnar, Senior Scientist, Environmental Virology Cell, NEERI says: “Swab collection method requires time. Moreover, since it is an invasive technique, it is a bit uncomfortable for patients. Some time is lost also in the transport of the sample to the collection centre. On the other hand, the Saline Gargle RT-PCR method is instant, comfortable and patient-friendly. Sampling is done instantly and results will be generated within 3 hours.”</p>
<p>
<strong>Samples can be collected by patients themselves</strong></p>
<p>
The method is non-invasive and so simple that the patient can himself or herself collect the sample, explains Dr. Khairnar. “Collection methods like nasopharyngeal and oropharyngeal swab collection require technical expertise; they are also time-consuming.</p>
<p>
In contrast, the Saline Gargle RT-PCR method uses a simple collection tube filled with saline solution. The patient gargles the solution and rinses it inside the tube. This sample in the collection tube is taken to the laboratory where it is kept at room temperature, in a special buffer solution prepared by NEERI. An RNA template is produced when this solution is heated, which is further processed for Reverse Transcription Polymerase Chain Reaction (RT-PCR). This particular method of collecting and processing the sample enables us to save on the otherwise costly infrastructural requirement of RNA extraction. People can also test themselves, since this method allows self-sampling.” The method is environment-friendly as well, since waste generation is minimized.</p>
<p>
<strong>A Boon for Testing in Rural Areas</strong></p>
<p>
The scientist expects that this innovative testing technique will be especially beneficial for rural and tribal areas where infrastructure requirements can be a constraint. The non-technique has received the approval of the Indian Council of Medical Research (ICMR). NEERI  has further been asked to train other testing labs, to help scale up its adoption across the country.</p>
<p>
Nagpur Municipal Corporation has given permission to go ahead with the method, following which testing has begun at NEERI, as per approved testing protocol.</p>
<p>
Dr. Khairnar and his team hopes that the method is implemented at the national level, resulting in faster and more citizen-friendly testing, thereby strengthening our battle against the pandemic.</p>